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squamous cell line ect1 e6e7  (ATCC)


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    ATCC squamous cell line ect1 e6e7
    The inhibition of L. crispatus -derived D-LA on FOXD1 expression and growth <t>of</t> <t>Ect1/E6E7</t> cells. A . immunofluorescence staining for HPV16 E7 protein in Ect1/E6E7 cells after 24-hour treatment with L. crispatus or L. gasseri culture supernatants, or D-LA. Nuclei are counterstained with DAPI (blue). Scale bar = 50 μm. B . HPV16 fluorescence intensity after treatments (mean±SD, n = 3; ** p < 0.01). C . CCK-8 assay measuring cell vitality after treatments (mean±SD, n = 3; ** p < 0.01). D . qPCR analysis of FOXD1 mRNA levels relative to GAPDH (mean±SD, n = 3; * p < 0.05, ** p < 0.01). Four co-culture system: without additives (control), with L. crispatus culture supernatant ( L. crispatus group), with L. gasseri culture supernatant ( L. gasseri group), or with purified D-LA (D-LA group)
    Squamous Cell Line Ect1 E6e7, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 352 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/squamous+cell+line+ect1+e6e7/Ect1%2FE6E7/pmc13085617-104-4-12
    Average 96 stars, based on 352 article reviews
    squamous cell line ect1 e6e7 - by Bioz Stars, 2026-10
    96/100 stars

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    1) Product Images from "Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus -derived D-lactic acid with cervical gene expression and precancerous cervical lesions"

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus -derived D-lactic acid with cervical gene expression and precancerous cervical lesions

    Journal: Journal of Translational Medicine

    doi: 10.1186/s12967-026-07982-w

    The inhibition of L. crispatus -derived D-LA on FOXD1 expression and growth of Ect1/E6E7 cells. A . immunofluorescence staining for HPV16 E7 protein in Ect1/E6E7 cells after 24-hour treatment with L. crispatus or L. gasseri culture supernatants, or D-LA. Nuclei are counterstained with DAPI (blue). Scale bar = 50 μm. B . HPV16 fluorescence intensity after treatments (mean±SD, n = 3; ** p < 0.01). C . CCK-8 assay measuring cell vitality after treatments (mean±SD, n = 3; ** p < 0.01). D . qPCR analysis of FOXD1 mRNA levels relative to GAPDH (mean±SD, n = 3; * p < 0.05, ** p < 0.01). Four co-culture system: without additives (control), with L. crispatus culture supernatant ( L. crispatus group), with L. gasseri culture supernatant ( L. gasseri group), or with purified D-LA (D-LA group)
    Figure Legend Snippet: The inhibition of L. crispatus -derived D-LA on FOXD1 expression and growth of Ect1/E6E7 cells. A . immunofluorescence staining for HPV16 E7 protein in Ect1/E6E7 cells after 24-hour treatment with L. crispatus or L. gasseri culture supernatants, or D-LA. Nuclei are counterstained with DAPI (blue). Scale bar = 50 μm. B . HPV16 fluorescence intensity after treatments (mean±SD, n = 3; ** p < 0.01). C . CCK-8 assay measuring cell vitality after treatments (mean±SD, n = 3; ** p < 0.01). D . qPCR analysis of FOXD1 mRNA levels relative to GAPDH (mean±SD, n = 3; * p < 0.05, ** p < 0.01). Four co-culture system: without additives (control), with L. crispatus culture supernatant ( L. crispatus group), with L. gasseri culture supernatant ( L. gasseri group), or with purified D-LA (D-LA group)

    Techniques Used: Inhibition, Derivative Assay, Expressing, Immunofluorescence, Staining, Fluorescence, CCK-8 Assay, Co-Culture Assay, Control, Purification

    Related Articles

    Inhibition:

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus -derived D-lactic acid with cervical gene expression and precancerous cervical lesions
    Article Snippet: The human immortalized cervical squamous cell line Ect1/E6E7 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).. Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus-derived D-lactic acid with cervical gene expression and precancerous cervical lesions.
    Article Snippet: concentration within bacterial supernatant was quantified by using the D-LA assay kits (Elabscience, China). .. Co-culture with Ect1/E6E7 cells The human immortalized cervical squamous cell line Ect1/E6E7 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA). .. Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.

    Derivative Assay:

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus -derived D-lactic acid with cervical gene expression and precancerous cervical lesions
    Article Snippet: The human immortalized cervical squamous cell line Ect1/E6E7 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).. Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus-derived D-lactic acid with cervical gene expression and precancerous cervical lesions.
    Article Snippet: concentration within bacterial supernatant was quantified by using the D-LA assay kits (Elabscience, China). .. Co-culture with Ect1/E6E7 cells The human immortalized cervical squamous cell line Ect1/E6E7 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA). .. Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.

    Expressing:

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus -derived D-lactic acid with cervical gene expression and precancerous cervical lesions
    Article Snippet: The human immortalized cervical squamous cell line Ect1/E6E7 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).. Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus-derived D-lactic acid with cervical gene expression and precancerous cervical lesions.
    Article Snippet: concentration within bacterial supernatant was quantified by using the D-LA assay kits (Elabscience, China). .. Co-culture with Ect1/E6E7 cells The human immortalized cervical squamous cell line Ect1/E6E7 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA). .. Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.

    Immunofluorescence:

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus -derived D-lactic acid with cervical gene expression and precancerous cervical lesions
    Article Snippet: The human immortalized cervical squamous cell line Ect1/E6E7 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).. Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus-derived D-lactic acid with cervical gene expression and precancerous cervical lesions.
    Article Snippet: concentration within bacterial supernatant was quantified by using the D-LA assay kits (Elabscience, China). .. Co-culture with Ect1/E6E7 cells The human immortalized cervical squamous cell line Ect1/E6E7 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA). .. Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.

    Staining:

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus -derived D-lactic acid with cervical gene expression and precancerous cervical lesions
    Article Snippet: The human immortalized cervical squamous cell line Ect1/E6E7 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).. Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus-derived D-lactic acid with cervical gene expression and precancerous cervical lesions.
    Article Snippet: concentration within bacterial supernatant was quantified by using the D-LA assay kits (Elabscience, China). .. Co-culture with Ect1/E6E7 cells The human immortalized cervical squamous cell line Ect1/E6E7 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA). .. Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.

    Fluorescence:

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus -derived D-lactic acid with cervical gene expression and precancerous cervical lesions
    Article Snippet: The human immortalized cervical squamous cell line Ect1/E6E7 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).. Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus-derived D-lactic acid with cervical gene expression and precancerous cervical lesions.
    Article Snippet: concentration within bacterial supernatant was quantified by using the D-LA assay kits (Elabscience, China). .. Co-culture with Ect1/E6E7 cells The human immortalized cervical squamous cell line Ect1/E6E7 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA). .. Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.

    CCK-8 Assay:

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus -derived D-lactic acid with cervical gene expression and precancerous cervical lesions
    Article Snippet: The human immortalized cervical squamous cell line Ect1/E6E7 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).. Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus-derived D-lactic acid with cervical gene expression and precancerous cervical lesions.
    Article Snippet: concentration within bacterial supernatant was quantified by using the D-LA assay kits (Elabscience, China). .. Co-culture with Ect1/E6E7 cells The human immortalized cervical squamous cell line Ect1/E6E7 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA). .. Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.

    Co-Culture Assay:

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus -derived D-lactic acid with cervical gene expression and precancerous cervical lesions
    Article Snippet: The human immortalized cervical squamous cell line Ect1/E6E7 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).. Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus-derived D-lactic acid with cervical gene expression and precancerous cervical lesions.
    Article Snippet: concentration within bacterial supernatant was quantified by using the D-LA assay kits (Elabscience, China). .. Co-culture with Ect1/E6E7 cells The human immortalized cervical squamous cell line Ect1/E6E7 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA). .. Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.

    Control:

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus -derived D-lactic acid with cervical gene expression and precancerous cervical lesions
    Article Snippet: The human immortalized cervical squamous cell line Ect1/E6E7 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).. Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus-derived D-lactic acid with cervical gene expression and precancerous cervical lesions.
    Article Snippet: concentration within bacterial supernatant was quantified by using the D-LA assay kits (Elabscience, China). .. Co-culture with Ect1/E6E7 cells The human immortalized cervical squamous cell line Ect1/E6E7 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA). .. Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.

    Purification:

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus -derived D-lactic acid with cervical gene expression and precancerous cervical lesions
    Article Snippet: The human immortalized cervical squamous cell line Ect1/E6E7 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).. Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus-derived D-lactic acid with cervical gene expression and precancerous cervical lesions.
    Article Snippet: concentration within bacterial supernatant was quantified by using the D-LA assay kits (Elabscience, China). .. Co-culture with Ect1/E6E7 cells The human immortalized cervical squamous cell line Ect1/E6E7 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA). .. Ect1/E6E7 cells were starved in serum-free DMEM medium for 24 hours.



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    Image Search Results


    The inhibition of L. crispatus -derived D-LA on FOXD1 expression and growth of Ect1/E6E7 cells. A . immunofluorescence staining for HPV16 E7 protein in Ect1/E6E7 cells after 24-hour treatment with L. crispatus or L. gasseri culture supernatants, or D-LA. Nuclei are counterstained with DAPI (blue). Scale bar = 50 μm. B . HPV16 fluorescence intensity after treatments (mean±SD, n = 3; ** p < 0.01). C . CCK-8 assay measuring cell vitality after treatments (mean±SD, n = 3; ** p < 0.01). D . qPCR analysis of FOXD1 mRNA levels relative to GAPDH (mean±SD, n = 3; * p < 0.05, ** p < 0.01). Four co-culture system: without additives (control), with L. crispatus culture supernatant ( L. crispatus group), with L. gasseri culture supernatant ( L. gasseri group), or with purified D-LA (D-LA group)

    Journal: Journal of Translational Medicine

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus -derived D-lactic acid with cervical gene expression and precancerous cervical lesions

    doi: 10.1186/s12967-026-07982-w

    Figure Lengend Snippet: The inhibition of L. crispatus -derived D-LA on FOXD1 expression and growth of Ect1/E6E7 cells. A . immunofluorescence staining for HPV16 E7 protein in Ect1/E6E7 cells after 24-hour treatment with L. crispatus or L. gasseri culture supernatants, or D-LA. Nuclei are counterstained with DAPI (blue). Scale bar = 50 μm. B . HPV16 fluorescence intensity after treatments (mean±SD, n = 3; ** p < 0.01). C . CCK-8 assay measuring cell vitality after treatments (mean±SD, n = 3; ** p < 0.01). D . qPCR analysis of FOXD1 mRNA levels relative to GAPDH (mean±SD, n = 3; * p < 0.05, ** p < 0.01). Four co-culture system: without additives (control), with L. crispatus culture supernatant ( L. crispatus group), with L. gasseri culture supernatant ( L. gasseri group), or with purified D-LA (D-LA group)

    Article Snippet: The human immortalized cervical squamous cell line Ect1/E6E7 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).

    Techniques: Inhibition, Derivative Assay, Expressing, Immunofluorescence, Staining, Fluorescence, CCK-8 Assay, Co-Culture Assay, Control, Purification